| # | FIRST LADY OR GENTLEMAN | LIVED | MARRIED |
| 1 | Margaret (Wylie) Mellette | 1843–1938 | 1866–1896 |
| 2 | Martha (Frizzell) Sheldon | 1854–1942 | 1875–1898 |
| 3 | Annie (Chappell) Lee | 1851–1918 | 1872–1918 |
| 4 | Eunice (Slye) Herreid | 1859–1940 | 1881–1928 |
| 5 | Mary Ellen (Masten) Elrod | 1863–1943 | 1884–1935 |
| 6 | Lavinia (Robinson) Crawford | 1870–1959 | 1896–1944 |
| 7 | Florence (Albert) Vessey | 1862–1933 | 1882–1929 |
| 8 | Emilie (Beaver) Byrne | 1866–1965 | 1888–1927 |
| 9 | Lydia (Anderson) Norbeck | 1873–1961 | 1900–1936 |
| 10 | Harriet (Russell) McMaster | 1879–1969 | 1902–1968 |
| 11 | Gertrude (Bertleson) Gunderson | 1871–1956 | 1892–1933 |
| 12 | Sarah (Johnson) Bulow | 1885–1976 | 1921–1960 |
| 13 | Elizabeth Jane (Parliament) Green | 1877–1976 | 1899–1945 |
| 14 | Lorena “Rena” (McLain) Berry | 1879–1963 | 1905–1951 |
| 15 | Elizabeth (Ward) Jensen | 1895–1985 | 1925–1964 |
| 16 | Vera (Cahalan) Bushfield1 | 1889–1976 | 1912–1948 |
| 17 | Emily (Auld) Sharpe | 1883–1957 | 1918–1957 |
| 18 | Madge (Turner) Mickelson | 1903–2006 | 1927–1965 |
| 19 | Vivian (Walz) Anderson | 1911–1981 | 1937–1981 |
| 20 | June (Shakstad) Foss | 1919–1970 | 1942–19672 |
| 21 | Lorna (Buntrock) Herseth3 | 1909–1994 | 1937–1969 |
| 22 | Florence (Dexter) Gubbrud | 1914–2006 | 1939–1987 |
| 23 | Borghild Boe4 | 1906–1994 | |
| 24 | Patricia (Henley) Farrar | 1931–2015 | 1953–2015 |
| 25 | Nancy (Pankey) Kneip | 1938– | 1958–1987 |
| 26 | Anne (Geigel) Wollman | 1936– | 1958–2022 |
| 27 | Mary Dean (Thom) Janklow | 1943– | 1960–2012 |
| 28 | Linda (McCahren) Mickelson | 1941– | 1963–1993 |
| 29 | Patricia (Kilber) Miller | 1953– | 1993–2015 |
| 30 | Mary Dean (Thom) Janklow | 1943– | 1960–2012 |
| 31 | Jean (Vedvei) Rounds | 1956–2021 | 1978–2021 |
| 32 | Linda (Schmidt) Daugaard | 1953– | 1981– |
| 33 | Bryon Noem5 | 1969– | 1992– |
| 34 | Sandy (Murphy) Rhoden | 1957– | 1981– |
- Succeeded her husband in the U.S. Senate upon his death in 1948.
- Joe and June Foss divorced in 1967. Joe Foss subsequently married Donna “Didi” Wild Hall.
- Served as Secretary of State from 1973–79.
- Borghild Boe acted as hostess for her brother, Nils Boe, who was a bachelor.
- As the husband of the state’s first female governor, Bryon Noem was the first “First Gentleman.”